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Medical University of South Carolina UNIV - Research Specialist II - Surgery: Surgical Oncology in Charleston, South Carolina

FLSA: Hourly

Job Summary: The Department of Surgery, Division of Surgical Oncology is currently recruiting for a Research Specialist II.

Payscale Salary Range: UNIV-Band 5: $33,494.00 - $47,734.00 - $61,975.00 (min - mid - max)

Job Duties:

  • 35% - Perform work involving the use of immunological analysis or genetic analysis of human clinical samples, or the use of small laboratory animals including collecting fluids and tissues; administering drugs and cells; perform cellular, chemical, and DNA analysis of blood and tissue samples; techniques used will include flow cytometry and PCR. Will also participate in tumor experiments involving mice; specifically, will inject mice subcutaneously with tumor and monitor tumor growth biweekly; will oversee overlapping experiments and sacrifice mice at set time points. Will participate in COVID-19 related research including working with convalescent patient PBMC, serum, and other human tissues. - (Essential)

  • 30% - Maintain laboratory instruments, supplies, and equipment. Maintain laboratory notebooks/records related to protocols, policy and regulation. Will also oversee titration of antibody stock reagents; this will involve coordinating titration efforts with other laboratory members and preparing a consolidated list of recommended antibody titrations for the entire laboratory. Critical to the effort will be maintaining a hard copy laboratory notebook and also copies of all relevant material on the external lab sever. Will also do research to find cost effective reagents for the lab. Coordinate sending biological samples to collaborators. - (Essential)

  • 25% - Perform experiments investigating cellular and molecular mechanisms involved in immune cell function including samples from patients on clinical trials. Utilize cell and molecular biology techniques including but limited to, cell culture, molecular cloning and enzymatic assays; transfections and gene expression assays; protein, RNA and DNA isolation and separation; ELISA; real-time RTPCR; srRNA experiments; immunostaining and flow cytometry; proliferation and apoptosis assays' and immunohistochemistry. Will develop and perform assays to assess the recycling of cytokines into lymphocytes; These efforts will involve the design and execution of a range of experiments that lead to a better understanding of cytokine signaling. These efforts will directly contribute to the grant and publication efforts of the laboratory. - (Essential)

  • 10% - Analyze and graph out relevant data from various experiments including tumor growth and cytokine signaling experiments; for tumor experiments, will use graphpad prism software to analyze means as well as individual mice; for cytokine signaling assays, will analyze experiments in flowjo and transfer these data to powerpoint; applicant will also present these data during lab meeting. As part of this effort, prepare analyze and prepare data for external collaborators. These efforts will be critical for the preparation of grants and/or manuscripts for the laboratory which will ultimately help in obtaining additional external funding. Will also prepare publication quality figures. - (Essential)

Preferred Experience & Additional Skills: Knowledge of Microsoft Office (Word, Excel, PowerPoint, and Outlook) preferred.

Interested applicants should include a cover letter indicating why they are interested in this position and their long-term career goals. Preference will be given to individuals with a stellar undergraduate GPA.

Minimum Experience and Training Requirements: A bachelor's degree in chemistry, biology, or other natural, life or health care science and one year work experience directly related to the assigned research area.

Physical Requirements: Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32? F to 100? F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/20 vision, corrected. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous)