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Medical University of South Carolina UNIV - Research Specialist II - Microbiology and Immunology in Charleston, South Carolina

FLSA: Hourly

Job Summary: The Ferreira Lab at the Medical University of South Carolina and the Hollings Cancer Center aims to design and develop the next generation of engineered immune cell therapies for autoimmune disease and cancer. This Research Specialist II position will involve providing technical assistance to the Principal Investigator in human and mouse immunology research studies using techniques such as gene editing, flow cytometry, molecular biology, and mouse models.

Payscale Salary Range: UNIV-Band 5: $33,494.00 - $47,734.00 - $61,975.00 (min - mid - max)

Job Duties:

  • 35% - Basic immunology experimentation of human and mouse samples employing various techniques, including CRISPR/Cas9 gene editing, viral transduction, human and mouse cell isolation, cell culture, flow cytometry, ELISA, DNA and RNA isolation, qPCR, bacterial culture, and microscopy. - (Essential)

  • 25% - Handling/processing of samples from human subjects involving PBMC isolation, serum/plasma separation, magnetic cell enrichment and fluorescence-assisted cell sorting (FACS), cryopreservation of biological samples, and record keeping. - (Essential)

  • 20% - Perform routine maintenance of and experimentation in mouse models. These responsibilities include breeding and weaning, separation, routine blood and other biological sample collection, genotyping, and treatment using various agents via different routes. - (Essential)

  • 15% - Management of the lab activities. This includes routine preparation of common lab reagents and buffers, as well as ordering, receiving orders, preparation of shipments, and maintenance of inventory of laboratory supplies. - (Essential)

  • 5% - Additional duties assigned by the Principal Investigator. This will include preparation of lab protocols, slides, figures, and other presentations. - (Essential)

Preferred Experience & Additional Skills: Flow cytometry, CRISPR/Cas9 gene editing, viral transduction, human PBMC isolation, human and mouse cell culture, microscopy, mouse colony management, animal tissue collection, bacterial culture, DNA and RNA isolation, molecular cloning, gel electrophoresis, ELISA, qPCR, SDS-PAGE, etc.

Minimum Experience and Training Requirements: A bachelor's degree in chemistry, biology, or other natural, life or health care science and one year work experience directly related to the assigned research area.

Physical Requirements: Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32? F to 100? F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/20 vision, corrected. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous)